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Step-by-step: Harvesting Yeast Slurry

  • Drain the beer from the fermenter, but leave 0.5–1 cm of beer on top of the yeast cake.

  • Swirl the yeast into the remaining liquid to create a uniform slurry.

  • Sanitize a jar or bottle with a lid (e.g. 0.5–1 liter).

  • Carefully pour the slurry into the jar – avoid the darkest trub at the bottom.

  • Store in the fridge at 0–4 °C.

  • Let it settle and decant if you want to remove excess trub. The whiter layer in the middle is the healthy yeast!

  • Close the lid loosely for 12–24 hours (due to CO₂ buildup), then seal tightly.

  • Re-use up to 5-6 times. Maybe don't re-use yeast from a 10-14% beer, because the yeast can be stressed

Yeast Calculator (Reused Yeast Slurry)

Re-use Yeast Calculator

Guide: Reusing Yeast Slurry in Homebrewing

Reusing yeast from previous brews is an effective and economical method used by many homebrewers. Here's a practical and detailed guide on how to collect, assess, and reuse yeast slurry correctly. The guide covers both ale and lager.

1 When and how do you harvest yeast?

After fermentation is complete, the yeast settles at the bottom of your fermenter as a creamy yeast cake. The best time to harvest is after the beer has been transferred, while a small amount of liquid still covers the yeast:

  • Leave a little beer (0.5–1 cm) on top of the yeast

  • Swirl the yeast into the liquid to create a pourable "slurry"

  • Pour the slurry into a clean and sanitized jar or bottle with a tight-fitting lid

  • Store in the fridge at 0–4 °C

If the slurry is very thick, you can add a little boiled and cooled water to adjust the consistency.

Tips: Always use sanitized equipment. Avoid scooping from the very bottom where the dark brown trub layer sits.

2 How do you evaluate if the yeast is good?

  • Color: Healthy yeast slurry is beige to cream-colored. If it’s very brown (like peanut butter), it’s likely old or dead.

  • Smell: It should smell like fresh brewing yeast or bread. If it smells sour, rotten, like sulfur, acetone, or vinegar – discard it.

  • Consistency: It should be like thick porridge or yogurt. No mold, film, or weird lumps on top. If you see odd layers or discoloration – do not use it.

3 Slurry thickness: Thin, normal, or thick?

The thickness of the yeast slurry affects how many cells are present per mL and how much you need to use:

  • Thin slurry: Very liquid, like soup. Approx. 0.75 billion cells/mL

  • Normal slurry: Like a milkshake or thick yogurt. Approx. 1.2 billion cells/mL

  • Thick slurry: Like dough or wet cement, barely pourable. Approx. 2.0 billion cells/mL

Assessment: Look at the jar after it’s been in the fridge for 30–60 minutes:

  • Clear liquid on top

  • Lighter yeast in the middle. This is the part you want to use

  • Dark trub at the bottom

4 Storage and shelf life

  • Store in fridge (0–4 °C)

  • Fresh slurry (<1 week) can be used directly

  • Slurry up to 3–4 weeks old is still usable, but cells gradually lose viability

  • If older than 4 weeks, a starter is recommended

  • Discard slurry with bad smell, discoloration, or visible mold

CO₂ Warning: Yeast may build pressure in the first few days. Keep the lid loose or vent briefly each day before sealing tightly.

5 Using the yeast calculator

Use a yeast calculator to determine how much slurry to pitch into your next batch:

  • Enter measurement system (L or gallons)

  • Enter beer volume and OG (Original Gravity)

  • Select yeast type (ale or lager)

  • Select yeast age (viability)

  • Select slurry thickness

The calculator will tell you how many mL of slurry to add. Don’t worry about slightly overpitching – but avoid underpitching. A starter is recommended for strong or large beers.

6 Tips for reusing yeast

  • Never use yeast from an infected batch

  • Yeast from highly hopped or high-alcohol beers may be stressed

  • Label the jar with date, yeast type, and batch name

  • Use a notebook or digital log to track generations and observations

  • A maximum of 5–6 generations is recommended; then switch to fresh yeast

Conclusion

Reusing yeast is a fantastic way to save money and maintain consistency in your brewing. Keep it clean, assess the condition of the yeast, and use the yeast calculator for accurate pitching. With a bit of practice, it’ll become a regular part of your brewing ritual.

Cheers and good luck with your next batch!

Sources:

  • BeerCraftr: "Reusing Yeast" (https://beercraftr.com/reusing-yeast/)

  • Brew Your Own Magazine: "Washing and Reusing Yeast"

  • Helderberg Homebrew Club: "Harvesting and Storing Yeast Slurry"

  • Brulosophy: "Pitch Rate Experiments"

  • John Palmer, How to Brew (book)

  • MoreBeer: "Yeast Storage and Viability"

  • Beersmith: "Using a Yeast Starter"

  • BrewFather and Brewer’s Friend calculators

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